上海滬震實(shí)業(yè)有限公司
主營(yíng)產(chǎn)品: elisa試劑盒
重組蛋白廠家-活性蛋白定制-高活性重組蛋白
價(jià)格
訂貨量(盒)
¥2380.00
≥1
¥2080.00
≥5
¥1880.00
≥10
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經(jīng)營(yíng)模式
生產(chǎn)廠家
所在地區(qū)
上海市閔行區(qū)
主營(yíng)產(chǎn)品
重組蛋白廠家;活性蛋白定制; 高活性重組蛋白
[ PROPERTIES ]
Residues: Asp248~Asp492 (Accession # Q01201),with two N-terminal Tags, His-tag and T7-tag.
Host: E.coli
Subcellular Location: Nucleus; Centrosome.
Purity: > 95%
Endotoxin Level:<1.0EU per 1μg(determined by the LAL method).
Formulation:Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted isoelectric point: 5.9
Predicted Molecular Mass: 31.3kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the
targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
[ SEQUENCE ]
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its
sequence is listed below.
MGSS HHHHHH SSG LVPRGS H MASMTGGQQM GRGSEF -DPY NAGS LKNHQE VDMNVVRICF QASYRDQQGQ MRRMDPVLSE PVYDKKSTNT SELRICRINK ESGPCTGGEE LYLLCDKVQK EDISVVFSRA SWEGRADFSQ ADVHRQIAIV FKTPPYEDLE IVEPVTVNVF LQRLTDGVCS EPLPFTYLPR DHDSYGVDKK RKRGMPDVLG ELNSSDPHGI ESKRRKKKPA ILDHFLPNHG SGPFLPPSAL LPDPDFFSGT VSLPGLEPPG GPDLLDDGFA YD
[ IDENTIFICATION ]
[ IMPORTANT NOTE ]
The kit is designed for research use only, we will not be responsible for any issue if the kit wasused in clinical diagnostic or any other procedures.