乙醇脫氫酶 來源于釀酒酵母 

Alcohol Dehydrogenase from Saccharomyces cerevisiaelyophilized powder (contains buffer salts), ≥300 units/mg protein

別名: ADH, 乙醇:NAD+ 氧化還原酶, 醇脫氫酶 來源于酵母 

Caution
含鍵合型 β-NAD 和 β-NADH，不適于 β-NAD 和 β-NADH 的回收微量分析。如需購買用于該目的的乙醇脫氫酶，請聯系我司在線客服**員。

Preparation Note
Dissolves in water at a concentration of 1 mg/mL to form a clear to slightly hazy, colorless to faintly yellow colored solution.
Unit Definition
在 pH 8.8 和 25°C 下，一單位本品每分鐘可將 1.0μmol 乙醇轉化為乙醛。
Physical form
含不超過 2% 檸檬酸緩沖鹽的固體
Application
Alcohol dehydrogenase has been used along with lactic dehydrogenase for the enzymatic reduction of acetaldehyde using sodium(R,S)-[2-3H] lactate.[2] Ethanol concentration can be determined colorimentrically by monitoring the enzymatic reduction of NAD using alcohol dehydrogenase after preremoval of the aldehyde group.[1]

Biochem/physiol Actions
分子量為 141kDa 的四聚體包含了 4 個相同的亞基。每個亞基的活性位點都含有一個鋅原子。每個活性位點還含有 2 個反應性巰基以及一個組氨酸殘基。 
等電點：5.4-5.8
最適 pH：8.6-9.0 
底物：酵母乙醇脫氫酶與乙醇最易反應，它的反應活性隨著醇體積增大或減小而降低。與支鏈醇和仲醇的反應活性也非常低。
KM（乙醇）= 2.1×10-3M
KM（甲醇）= 1.3×10-1M
KM（異丙醇）= 1.4×10-1M
劑：能與游離巰基反應的化合物，包括 N-烷基馬來酰亞胺和碘乙酰胺。
鋅螯合劑的劑，包括 1,10-菲咯啉、 
8-羥喹啉、2,2′-聯吡啶以及硫脲。 
底物類似物劑，包括 β-NAD 類似物、嘌呤和嘧啶衍生物、氯乙醇以及氟乙醇。
吸光系數：E1% = 14.6（水，280nm）

TEST Specification
________________________________________________________________________
Appearance (Color) White to Light Yellow to Light Brown
Appearance (Form) Powder
Solubility (Color) Colorless to Faint Yellow
Solubility (Turbidity) Clear to Slightly Hazy
1 mg/ml in H2O
Water (by Karl Fischer) < 12.5 % _
Salt Content < 2 % _
Citrate buffer salts
% Protein (UV) > 90 _
units/mg protein > 300 _
Enzymatic Activity: One unit will convert 1.0 micromole of ethanol to acetaldehyde per min at pH 8.8 at 25 deg C.
mol/mol ADH < 5 _
Total NAD/NADH Content
ICP-Atomic Emission < 0.01 _
Trace Copper: wt% of enzyme protein
Recommended Retest Period -------------------------
4 years
Specification: PRD.2.ZQ5.10000028735