產(chǎn)品名稱：禽呼腸孤病毒PCR檢測試劑盒價格

編號：BJ-P4620

分類：PCR檢測試劑盒

產(chǎn)品僅用于科研實驗儲存條件：-20℃避光保存，避免反復(fù)凍融。

運輸：低溫、避光，快遞免費送貨上門。

需要自備的器材：

1．禽呼腸孤病毒PCR檢測試劑盒價格儀器：分析天平、離心機(jī)、熒光 PCR 擴(kuò)增儀、組織研磨器、-20 ℃冰箱、可調(diào)移液器（2 μL、20

μL、200 μL、1000 μL）。

2．耗材：熒光 PCR 專用反應(yīng)管、眼科剪、眼科鑷、生理鹽水、1.5 mL 經(jīng)焦碳酸二乙酯（DEPC）水

處理的滅菌離心管、吸頭（10 μL、200 μL、1000 μL）、滅菌雙蒸水。

準(zhǔn)備物品：

清理液（A）                                   毫升

染色液（t B）                                   微升

稀釋液（C）                                   毫升

溶解液（tD）                                   毫升

產(chǎn)品說明書                                   1份

實驗注意事項：

1）RT-PCR可以檢測組織、細(xì)胞、血液、細(xì)菌等很多材料，不同的樣本有不同要求，實驗前請充分溝通確認(rèn)實驗方案和樣本情況；

2）客戶盡可能提供實驗的背景信息、物種、基因準(zhǔn)確的名稱和ID號；

3）客戶盡量不要提供DNA或RNA樣品。

4）樣本保存于液氮或干冰，也可以保存于Trizol液中。

實時熒光定量PCR（quantitative real-time PCR，qPCR）是指在PCR反應(yīng)體系中加入熒光基團(tuán)，利用熒光信號積累實時監(jiān)測整個PCR進(jìn)程，最后通過標(biāo)準(zhǔn)曲線對未知模板進(jìn)行定量分析的方法。實時熒光定量PCR的化學(xué)原理包括探針類和非探針類兩類，探針類是利用與靶細(xì)胞序列特異性雜交的探針來指示擴(kuò)增產(chǎn)物的增加，非探針類是利用熒光染料或者特異性設(shè)計的引物來指示擴(kuò)增的增加。運用該技術(shù)可以對DNA、RNA樣品進(jìn)行定量（包括絕對定量和相對定量）和定性分析。

主要服務(wù)：DNA或RNA的絕對定量分析、基因表達(dá)差異分析、基因分型。

主要技術(shù)：引物設(shè)計、RNA提取、cDNA合成、qPCR。

操作方法：

1直接測定

2測定準(zhǔn)備

3背景對照測定

4樣品活性測定

5計算樣品活性

技術(shù)特點：

1準(zhǔn)確可靠，臨床雙盲對照試驗＞1000例，結(jié)果與金標(biāo)準(zhǔn)測序法比對，結(jié)果一致性大于99%。

2高靈敏：可檢測低至10ng的人基因組DNA。

3快速：整個檢測流程只需3小時。

4簡便：試劑盒提供預(yù)混好的試劑，產(chǎn)品僅用于科研實驗使體系配置操作簡便。

5防污染

6高特異性：雙重特異性組成，保證檢測結(jié)果的特異性和準(zhǔn)確性引物與DNA互補鏈結(jié)合必需完全配對，才能延伸。探針特異性與所檢測基因的PCR產(chǎn)物配對，在延伸中產(chǎn)生熒光。

5-Oxo-Zaleplon-d5規(guī)格含量：100mgChemical Name:5-Oxo-Zaleplon-d5Synonyms:N-[3-(3-Cyano-4,5-dihydro-5-oxopyrazolo[1,5-a]pyrimidin-7-yl)phenyl]-N-ethyl-acetamide-d5; Pyrazolo[1,5-a]pyrimidine, Acetamide-d5 Deriv.; CL 345905-d5Alternate CAS #:Unlabelled: 159225-99-7Molecular Formula:C??H??D?N?O?Appearance:Pale Yellow to Light Yellow Solid

Ogerin規(guī)格含量：2.5mgChemical Name:OgerinSynonyms:2-[4-Amino-6-[(phenylmethyl)amino]-1,3,5-triazin-2-yl]benzenemethanolCAS Number:1309198-71-7Molecular Formula:C??H??N?OMolecular Weight:307.35

(4-Oxo-cyclohexylmethyl)carbamic Acid tert-Butyl Ester規(guī)格含量：50mgChemical Name:(4-Oxo-cyclohexylmethyl)carbamic Acid tert-Butyl EsterCAS Number:809273-70-9Molecular Formula:C??H??NO?Molecular Weight:227.3Category:Building Blocks; Miscellaneous;

2-Oxa-6-Azaspiro[3.4]Octane規(guī)格含量：10mgChemical Name:2-Oxa-6-Azaspiro[3.4]OctaneCAS Number:220290-68-6Molecular Formula:C?H??NOMolecular Weight:113.16Category:Building Blocks; Miscellaneous;

7-Oxa-2-azaspiro[3.5]nonane Hemioxalate規(guī)格含量：100mgChemical Name:7-Oxa-2-azaspiro[3.5]nonane HemioxalateCAS Number:1408076-09-4Molecular Formula:2[C?H??NO] ? C?H?O?Molecular Weight:344.4Category:Building Blocks; Miscellaneous;

2-Oxo-2,3-dihydro-1H-1,3-benzodiazole-5-carbaldehyde規(guī)格含量：50mgChemical Name:2-Oxo-2,3-dihydro-1H-1,3-benzodiazole-5-carbaldehydeCAS Number:106429-59-8Molecular Formula:C?H?N?O?Molecular Weight:162.14Category:Building Blocks; Miscellaneous;

3-[(2-Oxopyrrolidin-1-yl)methyl]benzonitrile規(guī)格含量：50mgChemical Name:3-[(2-Oxopyrrolidin-1-yl)methyl]benzonitrileCAS Number:1016840-72-4Molecular Formula:C??H??N?OMolecular Weight:200.23Category:Building Blocks; Miscellaneous;

4-(5-Oxo-3-phenyl-4,5-dihydro-1H-pyrazol-1-yl)benzonitrile規(guī)格含量：50mgChemical Name:4-(5-Oxo-3-phenyl-4,5-dihydro-1H-pyrazol-1-yl)benzonitrileCAS Number:118049-54-0Molecular Formula:C??H??N?OMolecular Weight:261.27Category:Building Blocks; Miscellaneous;

13-Oxo-12-oxa-2,3,7-triazatricyclo[7.4.0.0,2,6]trideca-1(9),3,5,7-tetraene-5-carboxamide規(guī)格含量：50mgChemical Name:13-Oxo-12-oxa-2,3,7-triazatricyclo[7.4.0.0,2,6]trideca-1(9),3,5,7-tetraene-5-carboxamideCAS Number:1221722-40-2Molecular Formula:C??H?N?O?Molecular Weight:232.19Category:Building Blocks; Miscellaneous;

5-[2-Oxo-2-(pyrrolidin-1-yl)ethyl]imidazolidine-2,4-dione規(guī)格含量：100mgChemical Name:5-[2-Oxo-2-(pyrrolidin-1-yl)ethyl]imidazolidine-2,4-dioneCAS Number:1025448-38-7Molecular Formula:C?H??N?O?Molecular Weight:211.21Category:Building Blocks; Miscellaneous;

8-Oxo-3,7-diaza-bicyclo[4.2.0]octane-3-carboxylic Acid tert-Butyl Ester規(guī)格含量：2mgChemical Name:8-Oxo-3,7-diaza-bicyclo[4.2.0]octane-3-carboxylic Acid tert-Butyl EsterCAS Number:1251005-47-6Molecular Formula:C??H??N?O?Molecular Weight:226.27Category:Building Blocks; Miscellaneous;

1-Oxazol-5-yl-ethanone規(guī)格含量：50mgChemical Name:1-Oxazol-5-yl-ethanoneCAS Number:1263378-07-9Molecular Formula:C?H?NO?Molecular Weight:111.09Category:Building Blocks; Miscellaneous;

4-(2-oxopropyl)morpholine-3,5-dione規(guī)格含量：50mgChemical Name:4-(2-oxopropyl)morpholine-3,5-dioneCAS Number:1248462-62-5Molecular Formula:C?H?NO?Molecular Weight:171.15Category:Building Blocks; Miscellaneous;

4-(5-Oxomorpholin-3-yl)benzenecarbonitrile規(guī)格含量：5mgChemical Name:4-(5-Oxomorpholin-3-yl)benzenecarbonitrileCAS Number:1391211-17-8Molecular Formula:C??H??N?O?Molecular Weight:202.21Category:Building Blocks; Miscellaneous;

7-oxoazepane-2-carboxylic acid規(guī)格含量：50mgChemical Name:7-oxoazepane-2-carboxylic acidCAS Number:14668-76-9Molecular Formula:C?H??NO?Molecular Weight:157.16Category:Building Blocks; Miscellaneous;
禽呼腸孤病毒PCR檢測試劑盒價格CDl4 ELISA Kit種屬:SH-SY5Y

形態(tài):上皮細(xì)胞樣

培養(yǎng)方法

培養(yǎng)基:MEM-EBSS:MinimumEssentialMedium(MEMEagleswithEarle＇sBalancedSalts)10%FBS

生長特性:貼壁生長與懸浮生長混合型