重組人白介素IL-4(GMP級)Recombinant Human interleukin IL4 ，是一種分泌蛋白，屬于IL-4 / IL-13家族。

惠誠生物提供IL4以及各類細(xì)胞因子，同時(shí)推出CHO穩(wěn)定細(xì)胞株開發(fā)技術(shù)服務(wù)方案，詳情咨詢電話021-60498804.

IL-4 Background Information:

Interleukin-4, also known as IL4, is a secreted protein which belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. It enhances both secretion and cell surface expression of IgE and IgG1. Interleukin-4 / IL4 also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Interleukin-4 is essential for the switching of B cells to IgE antibody production and for the maturation of T helper (Th) cells toward the Th2 phenotype. It participates in at least several B-cell activation processes as well as of other cell types. However, studies show that double mutant (Q116D, Y119D) of the murine IL4 protein (QY), both glutamine 116 and tyrosine 119, which binds to the IL4 receptor alpha, completely inhibites in a dose-dependent manner the IL4-induced proliferation of lipopolysaccharide-stimulated murine splenic B-cells, of the murine T cell line CTLL-2, and of the murine pre-B-cell line BA/F3. QY also inhibited the IL4-stimulated up-regulation of CD23 expression by lipopolysaccharide-stimulated murine splenic B-cells and abolished tyrosine phosphorylation of the transcription factor Stat6 and the tyrosine kinase Jak3 in IL4-stimulated BA/F3 cells.

產(chǎn)品說明：

白細(xì)胞介素-4（白介素-4, Interleukin-4, IL-4）是II型輔助T細(xì)胞（Th2細(xì)胞）分泌的細(xì)胞因子。白細(xì)胞介素-4的生物作用，包括刺激活化B細(xì)胞和T細(xì)胞增殖、CD4+T細(xì)胞分化成II型輔助T細(xì)胞，它也在調(diào)節(jié)體液免疫和適應(yīng)性免疫中起關(guān)鍵作用。白細(xì)胞介素-4誘導(dǎo)B細(xì)胞抗體類別轉(zhuǎn)換向IgE，上調(diào)第二型主要組織兼容性復(fù)合體的產(chǎn)生。在人類IL-4主要由活化T細(xì)胞產(chǎn)生。在小鼠由Th2亞群產(chǎn)生。此外，肥大細(xì)胞、IL-2刺激小鼠T細(xì)胞系2.19、ConA刺激人Th克隆2F1、小鼠胸腺瘤EL-4細(xì)胞以及B細(xì)胞系CH12均能分泌IL-4。

生物作用：包括刺激活化B細(xì)胞和T細(xì)胞增殖、CD4+T細(xì)胞分化成II型輔助T細(xì)胞，它也在調(diào)節(jié)體液免疫和適應(yīng)性免疫中起關(guān)鍵作用。白細(xì)胞介素-4誘導(dǎo)B細(xì)胞抗體類別轉(zhuǎn)換向IgE，上調(diào)第二型主要組織兼容性復(fù)合體的產(chǎn)生。此外，IL-4對于B細(xì)胞、T細(xì)胞、肥大細(xì)胞、巨噬細(xì)胞和造細(xì)胞都有免疫調(diào)節(jié)作用。

本產(chǎn)品由真核細(xì)胞表達(dá)的重組蛋白，是將蛋白基因接入穩(wěn)定表達(dá)載體，轉(zhuǎn)化CHO-K1細(xì)胞，經(jīng)克隆篩選，質(zhì)量驗(yàn)證，挑選的穩(wěn)定表達(dá)株，由化學(xué)成分確定的培養(yǎng)基（不含有任何動(dòng)物源成分）發(fā)酵制備，工藝穩(wěn)定，保證了產(chǎn)品質(zhì)量及批次間的一致性。

本產(chǎn)品為無菌凍干粉劑，由含有10mMPBS pH為7.2的蛋白溶液經(jīng)0.2μm過濾后分裝凍干。 

規(guī)格參數(shù)：

貨號：GMP-026     規(guī)格：50μg/1mg.

質(zhì)量控制：

生物學(xué)活性：大于5.0 x 106IU/mg.

純      度：經(jīng)高效液相色譜（SEC-HPLC）和SDS-PAGE檢測，純度大于95.0%.

內(nèi)  毒  素：小于5EU/mg.

使用說明：

建議將凍干rHuIL-4溶解在注射用水或滅菌的超純水中，濃度不低于100μg/ml，以待進(jìn)一步稀釋至工作濃度。避免反復(fù)凍融。

復(fù)溶后的細(xì)胞因子：4℃可穩(wěn)定儲存7-10天；長期保存請分裝后存放于-20℃。

本產(chǎn)品僅供研究使用/For Research Use Only.

上?；菡\生物科技有限公司成立于2010年，歷經(jīng)多年的積累，現(xiàn)推出CHO穩(wěn)定細(xì)胞株開發(fā)技術(shù)服務(wù)方案，方案涉及到的產(chǎn)品以及具體方案.歡迎來電咨詢：021-60498804

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