Polysciences(PEI)線性化聚乙希亞胺-PEI-MAX-40K
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Polyethylenimine HCl MAX, Linear, Mw 40,000
線性化聚乙烯亞胺鹽酸鹽MAX(PEI 40,000)
基本描述:
Polyethylenimine HCl MAX, Linear, Mw 40,000(PEI MAX 40K,PEI 40,000),不含鹽形式也稱為PEI 22K,是一種強大的,值得信賴且非常有效的瞬時轉染試劑。在HEK293和CHO表達系統(tǒng),在各種生產(chǎn)規(guī)模(從96孔板到100L生物反應器),PEI都提供連續(xù)性的高基因表達水平。每年都有更多的研究者和公司使用Polysciences PEI在關鍵工作中獲得優(yōu)勢。與大多數(shù)其他選擇相比,使用PEI配制轉染試劑在整個轉染實驗降低多達40%的開銷。
Polyethylenimine HCl MAX, Linear, Mw 40,000(PEI 40,000;PEI MAX 40K)是熱賣產(chǎn)品線性化聚乙烯亞胺PEI25,000(PEI 25K)的升級產(chǎn)品,不同之處在于1)完全水解(去乙?;?;2)PEI25,000的鹽酸鹽形式,具更高的水溶特性;3)含更長連續(xù)性乙烯亞胺(ethyleneimine)片段,其質子化氮水平比PEI25,000提高11%以上。本品經(jīng)堿中和后,可轉化為線性化自由胺(PEI),此時分子量正相當于25,000。
Thomas M,et al(2005)研究數(shù)據(jù)表明,去乙?;腜EI 40,000(Polyethylenimine MAX 40,000)體外質粒DNA轉染效率提高21倍,小鼠體內(nèi)靶向效率達到10,000倍,隨之肺部特異療效顯示增強1500倍。
PEI MAX 40K的優(yōu)勢:
1. PEI MAX 40K使用更方便:PEI 25K轉染溶液通常需幾個小時來配置,然而,PEI MAX 40K在2個小時內(nèi)即可轉化為即用型的溶液。
2. 與PEI 25相比,PEI MAX 40K連續(xù)性提供更高滴度【見Fig 1】:PEI 25含4-11%殘留的丙酰基基團,該基團阻止聚合物骨架緊密結合到DNA。然而,PEI MAX 40K完全去丙酰化,意味著每個批次一貫具更高轉化效率。
Fig 1. Comparison of PEI 25K (#23966) and PEI MAX 40K (#24765).Method: 10e6/mL HEK293 cells in 50 mL FS transfected with IgG64 plasmid pair. PEI:DNA 4:1. Samplestaken 120 hpt. Quantified with Thermo Fisher #23310. N=4 each. Error bar = standard deviation.
化學性質:
S NO. | 26338-45-4, 9002-98-6, 26913-06-4 |
Molecular Weight | 40,000(~22,000 free base) |
Solubility | Soluble In: Cold and room temperature water Insoluble In:Common organic solvents (ethanol, acetone, tetrahydrofuran) |
Appearance | White to off-white free flowing solid |
應用文獻【基因轉染用途】:
Mann JF, McKay PF, Arokiasamy S, Patel RK, Klein K, Shattock RJ. (2013).Pulmonary delivery of DNA vaccine constructs using deacylated PEI elicits immune responses and protects against viral challenge infection. J Control Release. 170(3):452-9.
Thomas M, Lu JJ, Ge Q, Zhang C, Chen J, Klibanov AM. (2005).Full deacylation of polyethylenimine dramatically boosts its gene delivery efficiency and specificity to mouse lung.Proc Natl Acad Sci U S A.102(16):5679-84.
Kobayashi, S., Yoshii, K., Hirano, M., Muto, M. Kariwa, H. A novel reverse genetics system for production of infectious West Nile virus using homologous recombination in mammalian cells. Journal of VirologicalMethods 240, 14–20 (2017). doi:10.1016/j.jviromet.2016.11.006
Longo, P. a, Kavran, J. M., Kim, M. Leahy, D. J. Transient Mammalian Cell Transfection withPolyethylenimine (PEI). Methods Enzymology 529, 227–240 (2013). doi:10.1016/B978-0-12-418687-3.00018-5.
Baranyi, L. et al. Rapid Generation of Stable Cell Lines Expressing High Levels of Erythropoietin, Factor VIII, and an Antihuman CD20 Antibody Using Lentiviral Vectors. Human Gene Therapy Methods 24, 214–227(2013). doi:10.1089/hgtb.2013.002
Kami, D. et al. Application of magnetic nanoparticles to gene delivery. International Journal of MolecularSciences 12, 3705–3722 (2011). doi:10.3390/ijms12063705
Wulhfard, S., Baldi, L., Hacker, D. L. Wurm, F. Valproic acid enhances recombinant mRNA and proteinlevels in transiently transfected Chinese hamster ovary cells. Journal of Biotechnology 148, 128–132 (2010). doi:10.1016/j.jbiotec.2010.05.003